There are 2 areas where you need to make choices on settings, the scanner settings in the main menu and the tool palette settings.
First, make sure your main menu settings are approprite for the film media you are scanning, e.g. slides, negatives, color or B&W. Refer to the manual if there are any questions here.
On the tool palette, no one can tell you what settings to use because it is determined by your image to be scanned. One rule I can tell you is never scan a B&W negative with ICE or DEE on, it will come out very poor. I have found the best process is to preview scan and then use the tool pallette settings to adjust as necessary while watching the effects under the modified tab. I have also found it much more preferable to do image adjustments in Photoshop rather than the Scan software.
Good luck, it's a long process.